ABSTRACT
A total of 60 herpes simplex virus (HSV) isolates obtained from individuals with genital herpes lesions in the mouth or respiratory illness were typed in this study. The HSV isolation rate in genital herpes was 31%. The typing was done by a direct fluorescent monoclonal antibody technique using commercial reagents. All the 60 Vero cell grown isolates were successfully typed without any indeterminate results. Nine isolates were typed as HSV 1 and 51 as HSV 2. The technique was easy to perform and read. HSV 2 was found to predominate in genital infections and HSV 1 in respiratory infections.
Subject(s)
Fluorescent Antibody Technique , Herpes Genitalis/microbiology , Herpes Simplex/microbiology , Humans , Respiratory Tract Infections/microbiology , Serotyping , Simplexvirus/classification , Stomatitis, Herpetic/microbiologyABSTRACT
Fifty-two paired sera and 40 single sera and swabs from the aphthous like ulcers of the tongue, gum, and buccal mucosa were collected from patients at a private clinic and from workers of canned fish packaging company in an attempt to isolate herpes simplex virus. Sixty-eight percent had positive HVH isolation with high ELISA antibody titers. Among 52 paired sera from patients, 68.4% had recurrent infections and 31.6% had primary infection.